The starting point that determines the existence of modern marketing is the objective existence of needs that are subject to some level of satisfaction. It is crucial for any tourism company to conduct marketing studies to determine its place in the modern tourism market and to identify potential users with the totality of their needs. In Bulgaria is located the SPA capital of the Balkan Peninsula - Velingrad, which combines a large number of SPA hotels, competing for a market share, more tourists and the sustainability of the offered tourist products and services. For these reasons, internet marketing is the key to the necessary information for tourist demand and supply, providing opportunities to achieve optimal management of tourism companies, associated with high financial results and effective positioning in relation to competition.
In the literatures, some podoscope systems have been introduced to analyze the plantar footprint. However, there is an absence of a low complexity portable system with control capacity and trace ability of users and their results through a web application. Therefore, the objective of this study is to develop of a portable system that allows the automation of the process; reducing the errors of human character and the times of accomplishment. \nThe development methodology of the research is a cascade type that begins as an initial phase in analyzing of the requirements and restrictions of the system. Then the physical and circuit design of the platform are carried out. Once the first two stages of the methodology are complete, the algorithms of the portable system based on Android Studio, and the web application are developed under the Laravel framework. Finally, the performance of the proposed system and the analysis of the results will be presented. In this study, the footprint of 35 users and a total of 70 samples will be analyzed. The results show 74% accuracy compared to the manual method.
From twenty bacterial isolates screened for their L-asparaginase activity, 16S rRNA sequencing for the most potent isolate showed that the selected isolate had 100% identity to pseudomonas aeruginosa strain (accession number: WCHPA075019). The maximum L-asparaginase productivity was achieved after 2 days, at 35°C, pH 7.6 under shaking at 200 rpm in the presences of L-asparagine (1%) and glucose (1%) as nitrogen and carbon sources, respectively. A low dose of gamma radiation (0.75 kGy) increases p. aeruginosa L-asparaginase production. Purification of L-asparaginase with 70% ammonium sulphate, followed by Sephadex G100 increases enzyme purity by 1.5-fold after gel filtration. Pure L-asparaginase had a molecular weight of 123 kDa by SDS- PAGE. The maximum enzymatic activity against L-asparagine was detected at 35°C and pH 9.0 after 30 min and 200 mM substrate. L-asparaginase activated in the presence of metal ions such as K+, and Na+, not affected when exposed to EDTA and strongly inhibited in the presence of Ba2+, and Cd2+. The anticancer activity of the purified enzyme was tested in vitro against three types of cell line carcinoma. The growth inhibition of HEPG2 carcinoma cell line by L-asparaginase was higher (IC50 value of 3.5 µg/ml) than the inhibition of HCT and MCF-7 carcinoma cell lines with IC50 value of 3.8 and 12.5 µg/ml, respectively relative to the growth of the untreated control cells. From the results, L-asparaginase from P. aeruginosa WCHPA075019 can be examined for medical applications as anticancer pharmaceutical agent for the tested cancer cell lines.
Gallstones occurs when the amount of cholesterol or bilirubin is high in bile and bile form solid particles in the gall bladder. Gallstones constitute a significant health problem and is a leading cause for hospital admission related to gastrointestinal problems. In majority of the cases the gallstone is silent and do not give any symptom such as acute cholecystitis, cholangitis or pancreatitis, however it is found as an incidental finding during abdominal ultrasound or by doing computed tomography scan. Cholelithiasis is a medico surgical problem for organ transplantation. In cholelithiasis the pain mechanism is usually obstruction located at gall bladder outlet, incoordination between gallbladder contraction and sphincter of oddi relaxation or visceral hypersensitivity. It may also be due to impaired gallbladder emptying, reliably quantitated by cholecystokinin cholescintigraphy. These are high chances of developing gall stones in chronic diseases like advanced cirrhosis, crohns disease, cystic fibrosis and sickle cell disease. Since Unani system of medicine is based on the health of humours (Ikhlat) Damwi, Balghami, Safrawi and Saudawi. The present work is an effort to establish association between risk factors and to observe the prevalence of gall-stone in social groups. The present study has been attempted with four different categories Damwi Bilious (Safravi) Phlegmatic (Balghami) and Saudawi temperament individuals because these two categories show different types of signs and symptoms as their physiological, physical, and psychological features and are opposite to each other on the basis of their Hot and Cold temperament respectively. Hence is has been observed that incidence of formation of gallstone is according to different body temperaments which is high in Safrawi (Choleric/ bileous) fallowed by Balghami (Phlegmatic), Damwi (Sanguine) and Saudawi (Melancholic).
In this study, the surface adhesion of the ink on composite materials was examined. For this purpose, composite materials were produced by adding polypropylene, thus polyethylene thermoplastic matrices were reinforced with sisal fibers. After the production of composites, the screen-printing process using water-based ink was applied to composite surfaces to investigate printing performance. As a result of these applications, the printability of the PE was found to be better than the PP. Also, the ink was adhered to the surface by forming a bond with the composite surface at polyethylene materials, but meanwhile not being able to hold onto the polypropylene composite surface.
The construction, development, and provision of more rural transport networks would not only boost the standard of living and reduce transport costs of the rural poor, but could also be a solution for the prolonged deplorable and worsening situation of rural roads in the country. This study examined the impact of rural transportation networks on farmers\' income in Ilaje local government area of Ondo State, Nigeria. A structured interview was used to gather information from a total of 120 farmers in rural communities across the local governments using a two-stage random sampling technique. Descriptive statistics and inferential statistics such as chi-square and Pearson Product Moment Correlation (PPMC) were used to analyze the data. The study revealed that farmers the poor nature of transportation networks have a negative impact on their income. Gender (χ2=6.472) and marital status (χ2=9.745) positively influenced income generated by farmers at p<0.05. Additionally, there was a moderate and significant correlation between transportation systems used (r=0.705, p<0.05), the perceived impact of rural transport (r=0.267, p<0.05) and the farmers\' income on agricultural activities. Based on the results of the study, it could be inferred that improved transport networks would enable farmers to work harder to increase productivity and reduce poverty in rural areas. The study recommended the development of motorable roads with sufficient infrastructure and the establishment of an agency or board capable of monitoring rural infrastructure to promote easy movement and improve the provision of medical services in the area.
Within the last decades, phytoplankton biomass has significantly risen in many lakes worldwide. Global warming was proposed as the most probable cause of the discovered effect. Here, we use a combination of paleolimnological, dendrochronological and meteorological datasets, as well as local pollution information, to analyze the recent growth of diatom total abundance in Lake Rabbvatn (69.7 N, 30.5 E; Northern Norway). The results show that Aulacoseira diatoms were most abundant in the top layers of the sediment core. On the contrary, the biomass of small-sized Cyclotella species which, as a rule, should grow simultaneously with a temperature warming, has decreased over the past decades. We suggest basing on the experimental data analysis that the recent growth of the total diatom abundance observed in Rabbvatn Lake, as well as in other lakes, could be mainly due to an increase in photosynthetically active spectral solar irradiance fluxes in the visible and infrared ranges. Further investigation concerning diatom assemblages in Arctic lakes seems to open new aspects of their application in light trapping nanotechnologies and paleoclimatology.